《植物生理学报》 2015, 51(7): 1075-1081
通信作者:郝丽珍;E-mail: haolizhen_1960@163.com;Tel: 0471-4318467
摘 要:
以室温贮藏1~8年的沙葱种子为试材, 研究贮藏陈化对其萌发、抗氧化系统及物质含量的影响。结果表明, 随贮藏时间延长, 萌发指标, 超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)活性, 可溶性蛋白质、糖和维生素C (VC)含量均为先升后降, 而丙二醛(MDA)含量、超氧阴离子(O2.–)产生速率和LOX活性为先降后升; 萌发后, O2.–产生速率、过氧化物酶(POD)和脂氧合酶(LOX)活性、可溶性蛋白质和VC含量逐渐降低, 而SOD、APX、CAT、GR活性和MDA、可溶性糖含量逐渐增加。萌发特性与SOD、CAT、GR活性和可溶性糖、可溶性蛋白质含量呈显著正相关, 与O2.–产生速率和LOX活性呈显著负相关, 贮藏过程中与MDA含量无显著相关性。关键词:沙葱; 种子; 贮藏陈化; 生理生化变化; 应答反应
收稿:2015-03-01 修定:2015-06-23
资助:农业部公益性行业科研专项经费项目(201203004)、国家自然科学基金(31260474、30660110)、内蒙古自然科学基金(2010MS0301、2015MS0362)。
Corresponding author: HAO Li-Zhen; E-mail: haolizhen_1960@163.com; Tel: 0471-4318467
Abstract:
The effects of storage ageing on germination index, antioxidant system and the content of several substances of the Allium mongolicum seeds stored for 1 year to 8 years were investigated. The results showed that the germination index, activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and glutathione reductase (GR), and contents of soluble protein, soluble sugar and vitamine C (VC) increased at first, then decreased with the extension of storage time. Malondialdehyde (MDA) content, superoxide anion radical (O2.–) generation rate and lipoxygenase (LOX) activity decreased firstly, then increased with the extension of storage time. After germination, the generation rate of O2.–, the activities of peroxidase (POD) and LOX, and the contents of soluble protein and VC decreased, but the activities of SOD, APX, CAT and GR and the contents of MDA and soluble sugar increased with the extension of germination time. The germination index had a significant positive correlation with the activities of SOD, CAT and GR and the contents of soluble sugar and protein, had a significant negative correlation with O2.– generation rate and LOX activity, and had no significant correlation with MDA content of the seed during storage.Key words: Allium mongolicum; seeds; storage aging; physiological and biochemical changes; responses
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